Inhibition of replication of T-tropic and M-tropic HIV-1 isolates by alloantigen-stimulated cell lines and supernatants. (A) PHA blasts were infected with HIV-1_BZ167 (▧, 172 TCID₅₀/10⁵ cells) or HIV-1_Ba-L(▪, 570 TCID₅₀/10⁵ cells) and cultured with an alloantigen-stimulated cell line, its supernatant (1:2 dilution), and the β-chemokines MIP-1, MIP-1β, and RANTES (200 ng/mL of each). p24 antigen production was assayed at 3 days post-infection by ELISA. Results are expressed as the percentage of p24 production by infected cultures in the absence of any treatment and represent means of triplicate cultures. (B) PHA blasts were infected with HIV-1_CD (▪) and HIV-1_EV (▧) (20 ng p24/10⁶ cells) and cultured with a supernatant derived from an alloantigen-stimulated cell line collected 4 days after alloantigenic stimulation. p24 production was assayed 3 days after infection by ELISA. Results are expressed as the percentage of p24 production by infected cultures in the absence of allostimulated supernatant. Results represent means ± SEM of two experiments performed in triplicate.