Effect of neutralizing anti-cytokine antibodies on the anti–HIV-1 activity of an allostimulated cell line and supernatant. (A) PHA blasts were infected with HIV-1_BZ167 (▪, 172 TCID₅₀/10⁵ cells) and HIV-1_Ba-L(▧,570 TCID₅₀/10⁵ cells) and cultured in the absence or presence of an allostimulated cell line. Cultures containing the alloantigen-stimulated cell line were also incubated with a pool of antichemokine (anti–MIP-1, –MIP-1β, and -RANTES) antibodies (50 μg/mL), anti–interferon-γ (25 μg/mL), or IgG control antibodies (50 μg/mL). HIV p24 antigen was measured on day 3 (HIV-1_BZ167) or day 6 (HIV-1_Ba-L) post-infection by ELISA. Results represent means of duplicate cultures from one experiment. (B) PHA blasts infected with HIV-1_BZ167 were cultured with an allostimulated supernatant in the presence or the absence of neutralizing anticytokine antibodies as above. HIV-1 p24 was measured on day 3 post-infection by ELISA. Results represent means of duplicate cultures from one of two separate experiments with similar results.