Fig. 2.
Fig. 2. Detection of allelic loss of the Fas gene in NHL by DGGE. A region encompassing the biallelic polymorphism, −670A/G, in the Fas promoter was amplified with primers FAS-734GC and FAS-623, and the two alleles were subsequently resolved by electrophoresis in a denaturing gradient gel. Unequal distribution of the two alleles was observed in DLC-B 128, anaplastic large cell lymphoma (ALCL) 72, and DLC-B 157, suggesting that oneFas allele was lost in the tumor cells. These three tumors harbor missense mutations in exon 6 or exon 7 of the Fas gene. In contrast, even distribution of the two alleles was observed in MALT 145, which harbors the N248K mutation in exon 9 of the Fasgene. PBL, DNA isolated from peripheral blood lymphocytes of a normal volunteer who is heterozygous for the −670A/G polymorphism.

Detection of allelic loss of the Fas gene in NHL by DGGE. A region encompassing the biallelic polymorphism, −670A/G, in the Fas promoter was amplified with primers FAS-734GC and FAS-623, and the two alleles were subsequently resolved by electrophoresis in a denaturing gradient gel. Unequal distribution of the two alleles was observed in DLC-B 128, anaplastic large cell lymphoma (ALCL) 72, and DLC-B 157, suggesting that oneFas allele was lost in the tumor cells. These three tumors harbor missense mutations in exon 6 or exon 7 of the Fas gene. In contrast, even distribution of the two alleles was observed in MALT 145, which harbors the N248K mutation in exon 9 of the Fasgene. PBL, DNA isolated from peripheral blood lymphocytes of a normal volunteer who is heterozygous for the −670A/G polymorphism.

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