Binding of endothelium to surfaces coated with fragments of soluble TF. Fragments representing different regions of TF were immobilized in microtiter wells. Endothelial cells were added in the presence or absence of anti-TF MoAb (VIC7, IVC6, IID8) or control MoAb (IgG). Cultures were incubated for 2 hours, washed, and the number of adherent cells was quantitated. The extent of binding to fragments was compared with the extent of binding to noncoated wells or wells coated with BSA (A). Photomicrographs of endothelial cells incubated for 2 hours in wells coated with a TF fragment representing amino acids 97-219 (B) or a fragment representing amino acids 1-122 (C). Photographs were taken before cultures were washed to remove nonadherent cells. These illustrate that TF fragment 97-219 supports spreading of EC, but fragment 1-122 does not. Original magnification × 150. Results are the mean of at least three independent experiments that were conducted using six replicates per parameter tested. HUVEC binding to fragments 1-219 and 97-219 was increased to a statistically signifcant degree over binding to control surfaces, P < .05. The block in binding to the full-length fragment by MoAb VIC7 and IID8 was also significantly lower than binding without addition of MoAb or in the presence of control IgG, P < .05.