Fig. 8.
Replating efficiency of wild-type and GATA-1.05mutant ES cell–derived hematopoietic progenitors. The adherent fraction cells contain hematopoietic progenitors that can form colonies on the OP9 feeder layer (CFU-OP9). The adherent fraction cells at day 14 were isolated and seeded on another OP9 layer. After 3 days in culture, colonies formed on OP9 were counted from three independentGATA-1.05 mutant ES cell lines and wild-type ES cells.