(A) Schematic diagram of the ribozyme Rzd cleavage sites and cleavage products. The closed arrows show the two sites of cleavage (a and b), whereas the open arrow indicates the end of the translated region (leu 157). (B) Cleavage of the 732 nt target TNF RNA with (+) or without (−) ribozyme Rzd. The data represent the PhosphorImager printout of 8% denaturing polyacrylamide gel. The arrows starting from the top indicate the cleavage fragments no. 1, 3, and 2 depicted in (A), respectively.