(A) Schematic diagram of a wedge of an agar plate. Sector 1 represents the center, whereas sector 5 represents the periphery of the plate. (B) Cleavage of the short substrate (site b) by the recovered ribozyme Rzd from each agar sector (1 through 5) as shown in (A). The arrow points to the cleavage products, whereas (−) represents a negative control.