Effect of accutin on HU VEC adhesion to immobilized ECMs. HUVECs (5 × 10⁴ cells/well) were subjected to 96-well plate, which were precoated with fibrinogen (40 μg/mL), vitronectin (10 μg/mL), or fibronectin (30 μg/mL), in the absence or presence of indicated concentrations of accutin (0.25, 1, 2 μmol/L) or GRGES (1 mmol/L). Results are expressed as percentage inhibition of adhesion compared with control cells in the absence of accutin. All experiments were conducted in quadruplicate and repeated at least three times. Data are presented as mean ± SEM (n = 3 to 6).