Fig. 2.
Effect of accutin on Matrigel-induced tube formation of HUVECs in vitro. HUVECs (1 × 105 cells/well) were plated on Matrigel in the presence of vehicle (A, control), Integrelin (B, 50 μmol/L), or accutin (C and D, 0.25 and 2 μmol/L, respectively) for 18 hours. After washing and fixation, cells were photographed under a phase-contrast microscope at 40× magnification.