Percentage inhibition of accutin on 7E3 binding to HUVEC. HUVECs were pretreated with GRGES (1 mmol/L) or indicated concentrations of accutin (0.068, 0.25, 1, 2 μmol/L) and with the primary antibody, 7E3 (20 μg/mL). After incubation with IgG-FITC (the second antibody), the mean fluorescence intensity of cells was determined by flow cytometry. Results are presented as percentage inhibition of adhesion compared with control cells (in the absence of accutin). Data are presented as mean ± SEM (n = 4).