Effect of 7E3 and GRGDS on FITC-conjugated accutin interaction with HUVEC. HUVECs pretreated with (A) antibodies, ie, 7E3 (b, 20 μg/mL), nonimmune IgG (c, 1:50 dilution) or (B) peptides (d, GRGDS and e, GRGES, both at 1 mmol/L) were incubated with FITC-conjugated accutin (1.3 μmol/L) and analyzed by flow cytomery. Nonspecific binding was performed by incubating cells with FITC-conjugated BSA (a in A and B). The tracing of PBS (control HUVECs) and that of GRGES pretreated HUVECs was almost identical. Similar results were obtained in at least four separate experiments. (C) Quantitative analyses of FITC-accutin and FITC-BSA were presented as mean fluorescence intensity and percentage of positively staining cells. Data are presented as mean ± SEM (n = 4). *P < .05 as compared with control.