Patterns of electrophoretic DNA fragmentation in accutin-treated HUVECs. HUVECs were incubated with vehicle (lane 1), GRGES (1 mmol/L, lane 2), or accutin (2 μmol/L, lane 3) for 18 hours and lysed. The total cellular DNA was isolated and subjected to electrophoretic separation by a 1.8% agarose gel. The internucleosomal DNA fragmentation was represented as the oligonucleosomal banding at lower molecular weight. A 100-bp ladder was shown in lane M. Similar results were obtained in three separate experiments.