Growth curve of 32Dcl3 cells in response to TGF-β in the presence IL-3 or G-CSF. (A) The indicated cells were cultured with 0.25 ng of IL-3 per mL, 10 ng of TGF-β per mL, and 10% FCS. Cultures were diluted when the cell number reached 1 × 106 cells per mL. Viable cells were counted by the trypan blue exclusion method at each time point. Clones B13 and B18 are established from cells transfected with pMV-AML1/Evi-1▵ZF8-10. Two independent experiments were performed and similar results were obtained. Representative data are shown. (B and C) The indicated cells were washed twice with phosphate-buffered saline and subsequently cultured with 5 ng of G-CSF per mL either alone (B) or together with 10 ng of TGF-β per mL (C) in the presence of 10% FCS. Cultures were diluted when the cell number reached 1 × 106 cells per mL.
