HPA-1 and HPA-3 typing on DNA and on mRNA from platelets from patient A.P., his mother (M.P.), and his father (F.P.). (A) SSCP analysis was performed on genomic DNA fragments. (B) Amplified cDNA was subjected to restriction enzyme digestion, HpaII for HPA-1 andFokI for HPA-3. The predicted size of the products is indicated. Analysis was performed on 10% polyacrylamide gels stained using EtBr (see arrows). M, size markers; UD, undigested fragment.