(A) Analysis of EHT gene in AML cases by quantitative Southern blot: cases AML11, 12, 22, and 24 show a loss of signal with respect to the control probe (PFL-1 probe for Bcl-2 gene). Because the percentage of leukemia cells in all cases is greater than 70%, this finding is consistent with an homozygous deletion. The probe (EHT3′) was specific for the 3′ UTR of EHT. (B) EHT gene expression analysis. Poly-A⁺ Northern blot filters (Clontech), were hybridized with the EHT3′ probe. The two dashes indicate the molecular weight (9.5 and 7.5 kb). The two transcripts were not represented in all of the tissues at the same level; in the testis, heart, brain, and skeletal muscle, the lower transcript was particularly expressed.