Fig. 5.
The suppression of the proliferative response against alloantigens in spleen cells from DST-treated and grafted animals is not donor-specific. Spleen cells were prepared from DST-treated and untreated heart graft recipients killed 5 days after the transplantation. Cells (2 × 105) were plated in triplicate alone with an equal number of Lew.1W (RT1.u) or third-party Buffalo (RT1.b) irradiated spleen cells or in the presence of 5 μg/mL of Concanavalin A (Con A) in 96-well round-bottom plates and cultured for 5 days. Thymidine incorporation was assessed during the last 8 hours. Representative data of five independent experiments are shown.
