American Society of Hematology

Fig. 2.

$Fig. 2. Morphology of newly formed dense cells. After 22 hours at pH 6.8 in 5% CO2-equilibrated buffer, as in Fig 1 (for B-F), cells were oxygenated for 30 minutes with 100% oxygen, fixed in glutaraldehyde, and examined by light microscopy. (A) Uncycled parent SS ND cells; (B) SS ND cells exposed to continuous oxygenation for 22 hours; (C) top density cell fraction (<1.10 g/mL) formed after 22 hours of O-D cycling; (D) bottom density cell fraction (>1.10 g/mL) formed after 22 hours of O-D cycling; (E) top density cell fraction (<1.10 g/mL) formed after 22 hours of continuous deoxygenation; and (F) bottom density cell fraction (>1.10 g/mL) formed after 22 hours of continuous deoxygenation.$

Morphology of newly formed dense cells. After 22 hours at pH 6.8 in 5% CO₂-equilibrated buffer, as in Fig 1 (for B-F), cells were oxygenated for 30 minutes with 100% oxygen, fixed in glutaraldehyde, and examined by light microscopy. (A) Uncycled parent SS ND cells; (B) SS ND cells exposed to continuous oxygenation for 22 hours; (C) top density cell fraction (<1.10 g/mL) formed after 22 hours of O-D cycling; (D) bottom density cell fraction (>1.10 g/mL) formed after 22 hours of O-D cycling; (E) top density cell fraction (<1.10 g/mL) formed after 22 hours of continuous deoxygenation; and (F) bottom density cell fraction (>1.10 g/mL) formed after 22 hours of continuous deoxygenation.

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