Fig. 2.
RT-PCR analysis for the expression of clone no. 3 (lane 1, molecular marker; lane 2, positive control consisted of PCR amplification of a recombinant plasmid containing sequence 3; lane 3, RT-PCR amplification of total RNA isolated from the primary leukemia cells; lane 4, negative control). Clone no. 3 PCR primer sequences were as follows: clone no. 3-1, 5′-CAA AGA AGA TCC TGA TGG-3′; clone no. 3-2, 5′-GCA GTG CAT TGG TCT ATC-3′.