Expression of CAR adenovirus receptor on HeLa cells; OCI-My5, RPMI 8226, ARH-77, HS Sultan, and IM-9 MM cells; patient MM1 cells; normal B splenocytes; CESS and JY EBV-transformed B cells; and normal BM MNCs. Cells (5 × 105/mL) were stained with RmcB anti-CAR MoAb (1:100) followed by 1 μL of FITC-conjugated goat (Fab′)2 antimouse IgG MoAb, or with 5 μL of FITC-conjugated mouse IgG1 (isotype control), and analyzed by indirect immunofluorescence flow cytometry. Cells examined included RD PCR3 (A; negative control) and RD CAR (B; positive control) cells; HeLa (C) cells, known to express adenovirus receptors; OCI-My5 (D), RPMI 8226 (E), ARH-77 (F), HS Sultan (G), and IM-9 (H) MM cells; patient MM1 (I) cells; normal B splenocytes (J); CESS (K) and JY (L) EBV-transformed B cells; and normal BM MNCs (M).
