Fig. 3.
Expression of vβ5 integrin and DF3/MUC1 on OCI-My5 and RPMI 8226 MM cells and on patient MM1 cells. OCI-My5 and RPMI 8226 MM cells and patient MM1 cells (5 × 105/mL) were stained with 1 μL of either vβ5 MoAb (A) or anti-DF3/MUC1 MoAb (B), followed by 1 μL of FITC-conjugated goat (Fab′)2antimouse IgG MoAb, or with 5 μL of FITC-conjugated mouse IgG1 alone (isotype control). The percentage of cells expressing these antigens was determined using indirect immunofluorescence flow cytometric analysis.