Inhibition of the differentiation of DC from CD34⁺ progenitors by RCC CM. CD34⁺progenitors were incubated with GM-CSF+TNF alone or in the presence of 10% of RCC CM or breast CM (control). Medium was replaced at days 6 and 10. A double staining with CD14-PE and CD1a-FITC antibodies was performed at day 6. At day 12, cells were labeled with CD14-PE/CD1a-FITC or CD86-PE/CD1a-FITC and analyzed on a FACScan (Becton Dickinson). Results are of one representative experiment performed with different seven CD34⁺ cells samples.