Dose-dependent inhibition of DC differentiation by RCC CM. Cells were cultured from day 6 to day 12 in the presence of an increasing percentage of RCC CM (1% to 20%; ⧫) or breast CM (○). At day 12, cells were harvested and processed for labeling with CD14-PE (—-) and CD1a-FITC (—) antibodies. Results are representative of five experiments.