Immunoblotting of K562 cells for band 3, 8% Laemmli gel. Lanes 1 and 6, K562/pBp; lanes 2 and 4, homozygous Memphis RBC ghosts; lanes 3 and 5, K562/B3 clone 1. BRIC 155 is a murine monoclonal antibody directed against an intracellular site at the C-terminus of band 3, while BRIC 170 binds to the N-terminal cytosolic domain of band 3.