Fig. 7.
Confocal z-sections of a NIH/3T3 cell transfected with KT3-epitope–tagged 4.1R80. NIH/3T3 cells were transfected with KT3-epitope–tagged 4.1-R80, fixed with 3% paraformaldehyde, permeabilized with 0.5% Triton X-100, and processed for immunofluorescence using a monoclonal anti–KT3-epitope tag antibody as a primary antibody and anti-mouse IgG coupled to Texas red as a secondary antibody. Cells were analyzed by confocal microscopy. The 4.1R80 isoform was strongly expressed in the nucleus, the plasma membrane, and apparent centrosomes. Every other z-section of a series of 18 0.5-μm z-sections are displayed. Scale bar: 10 μm.
