SSCP analysis of exon 11 of GPIIIa amplified from genomic DNA. Genomic DNA fragment of 198 bp comprising exon 11 of GPIIIa and intronic flanking regions was amplified as described in Materials and Methods. The PCR products were digested with HinfI to yield fragments of 140 and 58 bp and electrophoresed in nondenaturing 16% acrylamide slab gels containing 8.7% glycerol. The arrow points to a distinct band shown by the patient, her parents, and her brother that is absent in the control (wt) DNA.