Identification of a G¹⁸⁴⁶→T mutation in exon 11 of GPIIIa. Exon 11 of GPIIIa was amplified from genomic DNA as described in Materials and Methods. The amplification products were cloned in a T-vector and the primary nucleotide sequence of pooled DNA was determined in both directions. The figure shows a fragment of the sequencing ladder of the sense strand. The arrows point to a homozygous G¹⁸⁴⁶→T transversion that changes Glu⁶¹⁶→Stop.