Fig. 4.
![Fig. 4. Specific amplification of normal and [T1846]-GPIIIa alleles. A DNA fragment of 148 bp encompassing exon 11 and intronic flanking regions of GPIIIa was amplified from genomic DNA of a control, the proband, her parents, and her brother. Each DNA was amplified using a sense primer whose 3′ end was complementary to either the normal sequence (Wt) or to the mutant base (Mut). The amplification products were electrophoresed in a 3% agarose gel and the DNA bands were stained with ethidium bromide.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/12/10.1182_blood.v92.12.4712/5/blod42419004w.jpeg?Expires=1735526768&Signature=c3EBCxQWaYcEUOrIJEuDLEH4n2K4GrXRG1Ar4FqmO9j6N1z4w51I5dTgsJ2sIAtLFCx5stZZmCL58GrT8UP728XnRFQJxw~WlN7ljzppjEn6pIYkkTi60ag6KlyqvwO1PPIU7QMc4po4eWk2g6D6lj019i9HnG~4yignIgh02VfBXGTz7wU45VYwImz0GsatC4xoaaaijWZ~OwYBi~kUWgsHBAoNHkNVKvlmBTkQA2PzGuw6fsG7uSfna1c7mpmw1cBRRMK1qJYfIUx6WRmhV4Q1PDtl-MHGW8b~E4IYn7Oow9MiPRxW~pQCuwNzjmYxv2DZ0iu3dooYYRX48-Dz~w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specific amplification of normal and [T1846]-GPIIIa alleles. A DNA fragment of 148 bp encompassing exon 11 and intronic flanking regions of GPIIIa was amplified from genomic DNA of a control, the proband, her parents, and her brother. Each DNA was amplified using a sense primer whose 3′ end was complementary to either the normal sequence (Wt) or to the mutant base (Mut). The amplification products were electrophoresed in a 3% agarose gel and the DNA bands were stained with ethidium bromide.
