Representative immunophenotyping of chimeric NOD/SCID mouse BM 35 days after transplantation of MFG-EGFP/Am12 transduced, IL-3–, IL-6–, SCF–stimulated CD34⁺ UCB cells. BM (>10% CD45⁺) was stained with a panel of antibodies specific against different human blood cell lineages and CD45 as a marker for human cells. (A) The bright green autofluorescence on the x axes versus CD45. The window represents all human CD45⁺cells. The other dotplots shown are gated cells in this CD45⁺ window representing only human cells. Representative examples are shown for EGFP versus CD34 (B), EGFP versus CD33 (C), EGFP versus CD11b, (D) EGFP versus CD2 (E), EGFP versus CD4 (F), EGFP versus CD20 (G), and EGFP versus CD56 (H).