Fig. 3.
![Fig. 3. Treatment of neutrophils with the NADPH oxidase inhibitor DPI leads to an increase in caspase-3–like activity. Neutrophils undergoing spontaneous or Fas/APO-1–triggered apoptosis (▵ and □, respectively) were cultured in the absence or presence of DPI (10 μmol/L; ▴ and ▪) for various times (0 to 72 hours). Anti-Fas MoAb was used at 250 ng/mL. Determination of AMC release was performed in a fluorometric assay. The maximum rate of AMC release (pmol/min) was estimated by linear regression (r2 > 0.99). The mean and range of duplicate determinations from a representative experiment are shown. Solvent alone (ie, dimethyl sulfoxide [DMSO]) did not affect DEVD-AMC cleavage (data not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/12/10.1182_blood.v92.12.4808/5/blod42401003x.jpeg?Expires=1735841459&Signature=J3j3N~dvk9T6PNvIsavnbGESb4wC838ITgXzAYuIc1t5t~GkxBap7QGsINycO-0dBDuKqCi~6XwvVM63Qvn~YVBEUDXEfb~UozBiHd3D7GKa0CmOJZzSeD97OtHLesEuTsI-4xOVP7haNBpB1FqKSgEMypyxU~x5LGHcCEAjyxylVClrCWG6YZU-qudOea4YjHE0lusLbIwCYDbjjM7ZGfV-oNFbOgcSddj6KsixJ~svSJkKw8S4~EHDI7Ga6~SR4~SrgicgddIADbtAbuYgcjuuuZPirTNapQOQTyxEXSuFl-0830kuZeRta50CwlO9niCX3wPC9GLf7cZdYarsgw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Treatment of neutrophils with the NADPH oxidase inhibitor DPI leads to an increase in caspase-3–like activity. Neutrophils undergoing spontaneous or Fas/APO-1–triggered apoptosis (▵ and □, respectively) were cultured in the absence or presence of DPI (10 μmol/L; ▴ and ▪) for various times (0 to 72 hours). Anti-Fas MoAb was used at 250 ng/mL. Determination of AMC release was performed in a fluorometric assay. The maximum rate of AMC release (pmol/min) was estimated by linear regression (r2 > 0.99). The mean and range of duplicate determinations from a representative experiment are shown. Solvent alone (ie, dimethyl sulfoxide [DMSO]) did not affect DEVD-AMC cleavage (data not shown).
