Presentation of TT by DC generated using different culture conditions. 1.5 × 10⁵ PBMNC were incubated with 10⁴ irradiated APC with TT in 96-well plates. The absolute number of DC was determined by FACS staining for each culture condition. Each well was obtained after 7 days following a 12-hour pulse with tritiated thymidine. Results are expressed as mean cpm of triplicate wells ± SD. Thimidine incorporation of autologous T lymphocytes stimulated by DC and PBMNC not pulsed with TT: PBMNC, 724 ± 96 cpm; GM-CSF/IL-4, 1,244 ± 147 cpm; GM-CSF/IL-4/TNF-, 1,920 ± 141 cpm; GM-CSF/IL-4/CD40L, 1,770 ± 240 cpm; CD40L, 1,870 ± 96 cpm; CD40L/IL-4, 1,917 ± 366 cpm. (□) GM-CSF/IL-4/TNF-; (◊) GM-CSF/IL-4/CD40L; (•) GM-CSF/IL-4; (▵) CD40L; (⧫) CD40L/IL-4; (○) PBMNC.