Fig. 2.
Gel filtration chromatography of HS oligosaccharides following nitrous acid digestion. HS in HPLC peaks A and B from the nonsupportive cell PG (A and B) and in peak A from the supportive cell peak A PG (C) were subjected to low pH nitrous acid digestion and separated on a Sephadex G-25 column, as described in Materials and Methods. Fractions of 0.3 mL were collected and incorporated3H and 35S radioactivity was monitored.
