Angiostatin induces cytotoxicity in bovine and human endothelial cell types. Confluent monolayers of BME, BAE, BLE, HUVE, and HMEC-1 cells were treated with ehAst at the indicated concentrations, and endothelial cell cytotoxicity was assessed using the ethidium homodimer incorporation assay after 72 hours. Results are shown as the mean of duplicate samples.