Fig. 9.
Effect of VEGF infusion on colony efficiency of BM cells. Mice were treated with infusion of VEGF (50 ng/h; ▪) or PBS (control;
) for 3, 5, 7, or 28 days. After that time, BM cells were isolated and cultured in triplicate in semisolid methylcellulose medium supplemented with recombinant cytokines supporting growth of erythroid and myeloid colonies (Methocult GF M3434; Stem Cell Technologies). BFU-E colonies were scored on day 8 and the remaining colonies were evaluated on day 12-13. Each experiment included two mice per group. The number of colonies was calculated per 105 cells. Mean ± SE of two independent experiments is shown.
