Effect of VEGF treatment in vitro on progenitor cells. ECCM and AECCM were obtained as described in the text. BM cells were obtained from control mice and incubated for 2 hours with either 100 ng/mL VEGF or with 50% vol/vol ECCM or AECCM. After that time, cells were washed and used in further experiments. Each experiment included two mice per group. Mean ± SE of two independent experiments is shown. (A) Colonies in semisolid methylcellulose medium. Cells were cultured in triplicate in methylcellulose medium and the number of colonies per 105 BM cells were scored exactly as described in the legend to Fig 9. Cumulative results from all experiments are shown. Y-axis, the number of colonies per 105 cells. (B) Spleen colonies were scored based on morphological criteria. Cumulative results from all experiments are shown.
