Time course of elastase release from neutrophils adherent to platelets either (A) unstimulated and continuously rolling (□) or stimulated with 10−7 mol/L fMLP (▪); (B) stimulated with 1% ZAP as a source of C5a; or (C) stimulated with 1 ng/mL IL-8. Data are the mean ± SEM of least four experiments. Analysis of covariance showed that there was a significant effect of time and treatment on elastase release (P < .01) and that treatments with chemotactic agents were significantly different from control (P < .01), but not different from each other. One-way ANOVA showed that elastase release from control cells did not vary with time but that there was a significant effect of time on release for individual treatments with chemotactic agents (P < .05 in each case).
