RT-PCR analysis of transgene expression. RNA was prepared from various tissues of hG-CSFR-Tg mice in line #70. cDNA derived from 1 μg total RNA was used for PCR. The lane marked (−) is the PCR product of a mock cDNA (no reverse transcriptase included in the cDNA synthesis reaction). Normal mouse indicates the PCR product of the bone marrow. PCR was performed for 30 cycles. Expression of the hG-CSFR transgene was observed in BM, PB, spleen, thymus, liver, heart, intestine, brain, and kidney.