PRR2-Fc soluble receptor binds to PRR2 transfectants. (A) FACS analysis of Da-1 (continuous black) and Da-1/PRR2 lo (continuous gray), me (dashed black), and hi (dashed gray) stained with R2.477 MoAb. (B) FACS analysis of these transfectants stained with 2 μg of PRR2-Fc. Inserts show Western blot analysis of the PRR2-Fc soluble receptor using a peroxidase-conjugated goat antihuman Fc antibody and a plot corresponding to the MFI of PRR2-Fc binding as a function of the MFI of PRR2 expression.