Expression of c-kitD814H in ELM cells causes SCF-indpendence and enhanced proliferation and cloning efficiency. (A) The proliferation of ELM-Kit and ELM-KitDH cells over 7 days was compared after seeding 500 cells in 96-well plates in different concentrations of SCF. Cells were assayed using a colorimetric MTT proliferation assay and each data point shown is an average of 28 cultures, each read twice, with standard deviations. (B) Colony formation by ELM-Kit, ELM-KitDH, and ELM-I-1 cells in response to SCF. Five hundred cells were inoculated in the presence or absence of SCF (10 ng/mL) in semisolid medium.