Comparison of the effects of wild-type c-kit and c-kitD814H expression in ELM-D cells on (A) the pattern of Shc isoforms expression, (B) bcl-2 mRNA, and (C) Fli-1 protein. (A) Relative expression of Shc isoforms was investigated by Western blotting of whole cell lysates of serum-starved cells and ELM-Kit cells serum-starved in the presence of 10 ng/mL SCF. (B) Expression of bcl-2 mRNA in ELM-I-1, ELM-Kit maintained in 10 ng/mL SCF, and ELM-KitDH cells (clones i and ii) was analyzed by Northern blotting of total RNA from growing cells and probing with bcl-2 cDNA and 7S rRNA probes. Size markers are shown in kilobases. (C) Whole cell lysates from ELM-KitDH and ELM-I/1 cells or ELM-Kit cells maintained in SCF were analyzed by Western blotting with an anti-Fli-1 antibody.