Stat6 inhibits a minimal IL-4 promoter construct. Jurkat cells were cotransfected with a minimal promoter construct lacking the known P2 Stat6 element (pCAT 145) with or without a wild-type (wt) or DNA-binding domain mutant (DBD mut) Stat6 expression vector as indicated. Cells were stimulated for 18 hours with calcium ionophore (▧) with or without IL-4 as indicated, followed by cell lysis and analysis of reporter gene expression by ELISA. Results are expressed relative to CAT production in unstimulated cells and are the mean ± SEM of four (DBD mut) or seven (wt) independent experiments. *P< .05.