The P2 element contains overlapping binding sites for Stat6 and NFATp. (A) Alignment of the human IL-4 P2 NFAT element with canonical binding sites for NFAT (overline) and Stat6 (underline). Note that the 3′-end of the Stat6 sequence overlaps the 5′-end of the NFAT site. (B) The ability of recombinant Stat6 to interact with wild-type (wt) and mutated probes (see Materials and Methods) was determined by EMSA. Stat6 no longer bound the m2-m4 oligonucleotides, confirming the overlapping nature of the Stat6 and NFAT binding sites.