Stat6 binds competitively with NFAT to the P1 and P2 elements. (A) The ability of a recombinant fragment of NFATp (see Materials and Methods) to bind oligonucleotide probes containing the P1 and P2 elements in the presence of increasing concentrations of recombinant Stat6 was determined by EMSA. The relative mobilities of each factor are indicated. Serial twofold dilutions of Stat6 were examined against a constant amount of NFATp. n.s., nonspecific. (B) The relative intensities of observed bands were analyzed by densitometry and expressed relative to intensity of the NFAT complex for each oligonucleotide in the absence of Stat6 (which was defined as 1).