(A) Flow cytometric analysis of ₄ integrin and VCAM-1 expression in BAF-B03 cells and transfectants. The various cells were unstained (dotted line) or stained with anti-₄ integrin and anti–VCAM-1 MoAbs (solid line), followed by staining with FITC-labeled secondary antibodies and a flow cytometric analysis, as described in Materials and Methods. (B) Flow cytometric analysis of expression of various integrin subunits and ICAM-1 on BAF-B03 cells (□) and BMC cells (▪). The mean fluorescence intensity values are shown.