Fluorescence-imaged microdeformation (A through C) and the comparison of the difference in entrance and cap density for band 3 in positive and negative dog RBCs (D through F). (A through C) A bright field image of a micropipette-aspirated RBC (A), equivalent fluorescent image (B), and integrated fluorescence-density profile (C) used for the analysis. The collected density profiles from the negative (D) and positive RBCs (E) at 112 days post-biotinylation are plotted in (D) and (E), respectively. The composite data of ρ_e-ρ_c against L/R_p for positive and negative cells are shown in (F).