Effects of AT and various agents on serum levels of aminotransferases in rats after hepatic I/R. Animals were subjected to 60 minutes of hepatic ischemia followed by reperfusion as described in Materials and Methods. Animals were injected IV with AT (250 U/kg) 30 minutes before reperfusion or subcutaneously with IM (20 mg/kg) 30 minutes before ischemia. DEGR-Xa (3 mg/kg) or Trp⁴⁹-modified AT (250 U/kg) was injected intravenously 30 minutes before reperfusion. Iloprost was infused continuously starting just before the onset of ischemia at a rate of 100 ng/kg/min with or without subcutaneous injection of IM (20 mg/kg). Serum levels of ALT and AST were determined after 12 hours of reperfusion. Each bar represents the mean ± SD. *P < .01 versus sham. †P < .01 versus I/R. ‡P < .01 versus AT-treated I/R. ¶P < .01 versus IM-treated I/R.