Effects of AT, DEGR-Xa, and Trp⁴⁹-modified AT on the changes in hepatic tissue blood flow in rats subjected to hepatic I/R. Animals were subjected to 60 minutes of hepatic ischemia followed by reperfusion as described in Materials and Methods. Animals were injected IV with AT (250 U/kg), DEGR-Xa (3 mg/kg), or Trp⁴⁹-modified AT (250 U/g) at 30 minutes before reperfusion. Hepatic tissue blood flow was continuously measured by a laser Doppler flowmeter starting 30 minutes before ischemia until 3 hours after reperfusion. Each value represents the mean ± SD derived from 5 animals. (○) I/R animals; (•) AT-treated I/R animals; (▪) DEGR-Xa-treated I/R animals; (□) Trp⁴⁹-modified AT-treated animals. †P < .01 versus I/R. ‡P< .01 versus AT-treated I/R.