Fig. 2.
![Fig. 2. Internalization of surface-bound [125I] G-CSF by WT and mutant ▵716 G-CSFR complexes. Three days after transfection COS-7 cells were washed, incubated with 500 pmol/L [125I]-G-CSF for 2 hours at 4°C, then shifted to 37°C for the indicated times. Cells were returned to ice again for 2 hours, then washed to eliminate unbound ligand. Surface-bound ligand (▪) was determined after acid stripping in 0.5 mol/L NaCl/HCl (pH1.0) for 3 minutes. Internalized ligand (○) was measured after lysis of the cells in 1 mol/L NaOH. Data are expressed as a percentage of initial binding of G-CSF at 4°C. Values represent the mean of duplicate points. The data shown are from three independent experiments. Standard deviations are indicated by error bars.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/2/10.1182_blood.v93.2.440/4/blod40223002x.jpeg?Expires=1735518147&Signature=14If3DWPENT0bsrrv0vciwr5I5yErElhhrQxQtosJtLL-0GGJJ-tfbr8Az3m8QF~EbKPhKc3Sy6cbMqDkYv7yCepY4cHS~VbX-3oZLsGFFbkqEufB~K-mTxb9r72kg4l9SIM74Vc-m~8-vrMz7ScJW~1wIxoFLrqWKeToiSOUp6dNaPVZNy-ni1Vr99F0gkK0MWFrC2bwsdiSRW4cOGJ8-~TgNwo-zhmy2dqFyps9t4T-xKbORXgqp9nVuMZuplIOfnK1h6T8-AdOBbsR6nSBatAjwqZSd-NESwlXz-Fo4u5opeST9VRKnzTaSq5FoWg6cXD9WVB5ptweXMNtgSy1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Internalization of surface-bound [125I] G-CSF by WT and mutant ▵716 G-CSFR complexes. Three days after transfection COS-7 cells were washed, incubated with 500 pmol/L [125I]-G-CSF for 2 hours at 4°C, then shifted to 37°C for the indicated times. Cells were returned to ice again for 2 hours, then washed to eliminate unbound ligand. Surface-bound ligand (▪) was determined after acid stripping in 0.5 mol/L NaCl/HCl (pH1.0) for 3 minutes. Internalized ligand (○) was measured after lysis of the cells in 1 mol/L NaOH. Data are expressed as a percentage of initial binding of G-CSF at 4°C. Values represent the mean of duplicate points. The data shown are from three independent experiments. Standard deviations are indicated by error bars.
