Fig. 3.
![Fig. 3. Progeny of AFT024 cultures function to stimulate allogeneic mixed lymphocyte reactions. Monocyte-depleted peripheral blood mononuclear cells (105) from the same donor were mixed with irradiated (3,000 rads) autologous CD14+ fresh monocytes (▪), 6-week progeny of AFT024 cultures (•), and 5-week progeny of AFT024 cultures transferred for an additional week to media containing TNF, GM-CSF, and IL-4 (○). Proliferation (counts per minute [cpm]) was assessed after 5 days by pulsing for 18 hours with3H-thymidine and data are presented as the mean ± SEM cpm of triplicate wells from a representative experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/1/10.1182_blood.v93.1.96/5/blod40113003x.jpeg?Expires=1735522754&Signature=sDGDL9gleJRM2lubEaj~I1pv2SQRIzSF4rbCa2IAP1WQ4Ox-HnIWxd8YrRCm7eqtSmowEdJGkqCsgF65hanyxf5l2TNJax4T~Cmrs06ykMXkwl0lFAsVFt78gXBru~SMkAEhxKbRitnuOXfZ3DlePPC96W6H-Dm6D~89vQQO3OQB3hX2tvT7Np7Zagb867asU4QTquG57Doq8g1tM3b8Hus6lq~wYJqij6MhIKreDnPFz6kBu51wvDO3GqNVnLT~hQD4OBCwnIRY27ZEpoguQFZDsNduhs1wQhjCP5DHKS53jQYefTss-Sh9OIJiejFCQh8kKOGKYiDEFHLsumqtpQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Progeny of AFT024 cultures function to stimulate allogeneic mixed lymphocyte reactions. Monocyte-depleted peripheral blood mononuclear cells (105) from the same donor were mixed with irradiated (3,000 rads) autologous CD14+ fresh monocytes (▪), 6-week progeny of AFT024 cultures (•), and 5-week progeny of AFT024 cultures transferred for an additional week to media containing TNF, GM-CSF, and IL-4 (○). Proliferation (counts per minute [cpm]) was assessed after 5 days by pulsing for 18 hours with3H-thymidine and data are presented as the mean ± SEM cpm of triplicate wells from a representative experiment.
