Multiple lineages are present from the cultured progeny of single cells. Single CD34⁺/Lin⁻/CD38⁻ cells were cultured on AFT024 for 42 days with IL-7, FL, KL, IL-2, and IL-3 (A and B are from progeny of the same single cell) or with IL-7, FL, KL, and IL-3 (C and D are from progeny of the same single cell). Data are examples of the three-color phenotypes summarized in Table 2 showing the multilineage differentiation, which resulted from single cell cultures gated on all viable cells (panel A and C) or a smaller lymphocyte gate (panel B and D).