Comparision of DC numbers identified as either lin⁻ HLA-DR⁺ cells (before culture) or as CMRF44⁺ CD14⁻ CD19⁻ cells (after overnight culture). (A) The mean numbers of lin⁻HLA-DR⁺ cells and CMRF44⁺DC were both reduced in patients (n = 13) compared with normal individuals (n = 11). (B) The relatively constant relationship between the numbers of CMRF44⁺ DC and lin⁻HLA-DR⁺ cells seen in normal individuals (where CMRF44⁺ DC typically account for 40% to 80% of lin⁻ HLA-DR⁺ cells) is not seen in the patient group (CMRF44⁺ DC account for 5% to 95% of the lin⁻ HLA-DR⁺ cells). (C) The use of CMRF44 or CD83 to identify DC was compared in both normals (n = 5) and patients (n = 16). A strong correlation (r²= 0.89) was found between the DC numbers measured using CMRF44 or CD83.